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pbpG [2018-02-16 17:18:08]
penicillin-binding protein 2d
function
bifunctional glucosyltransferase/ transpeptidase
product
penicillin-binding protein 2d
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]Gene
Coordinates
3,849,818 → 3,851,893
The protein
[SW|Localization]
cell membrane (according to Swiss-Prot)prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary]Expression and Regulation
Operons
genes
[gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG]
description
[Pubmed|22383849]
sigma factors
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|16497325,15758244,10767540], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon][protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]regulation
expressed during sporulation in the forespore ([protein|search|SigF], [protein|search|SigG]) [Pubmed|16497325,15758244,10767540]additional information
A [protein|search|ncRNA] is predicted between '[protein|search|pbpG]' and '[protein|search|ywhD]' [PubMed|20525796]view in new tabBiological materials
Mutant
MGNA-A520 (ywhE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/520 NBRP B. subtilis, Japan]BKE37510 (Δ[gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAACGGGTTCCCCCTTTT, downstream forward: _UP4_TGAAAATAACCCGGCTCCTCBKK37510 (Δ[gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAACGGGTTCCCCCTTTT, downstream forward: _UP4_TGAAAATAACCCGGCTCCTCGFP fusion
3510 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG]::pSG5309 (cat Pxyl-gfpa-[gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG]1-544) [pubmed|15758244], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jeff Errington] labReferences
16497325,11567005,10767540,20525796,15758244